Comparison of Filtered and Direct RGB LED Illumination in Confocal Imaging for Cellular Imaging

Authors

  • Muhammad Faizul Hadi Abu Kasim Department of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia https://orcid.org/0009-0001-1932-4211
  • Fazliyana 'Izzati Za'abar Department of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia
  • Mohd Hadri Hafiz Mokhtar (1)Department of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia (2) Photonics Technology Research Group, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia
  • Norhana Arsad (1)Department of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia (2)Photonics Technology Research Group, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia
  • Mohd Norzaliman Mohd Zain Malaysian Institute of Microelectronic Systems, Bukit Jalil, 57000 Kuala Lumpur, Malaysia https://orcid.org/0000-0002-2645-2975

DOI:

https://doi.org/10.53655/joe.z7420y

Keywords:

Coloured illumination, RGB LED, white LED, confocal microscopy, image quality

Abstract

This study investigates the comparative performance of filtered and direct RGB LED illumination in confocal microscopy for biological imaging. Rotifera whole-mount samples were imaged under both illumination modes, and image quality was assessed using signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) metrics. The highest SNR was recorded under filtered green LED illumination, whereas the highest CNR was achieved with direct red LED light. These results indicate that filtered RGB illumination enhances overall image uniformity, while direct RGB lighting more effectively accentuates structural contrast, depending on the specific imaging objective.

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Published

02-11-2025

How to Cite

Muhammad Faizul Hadi Abu Kasim, Fazliyana ’Izzati Za’abar, Mohd Hadri Hafiz Mokhtar, Norhana Arsad, & Mohd Norzaliman Mohd Zain. (2025). Comparison of Filtered and Direct RGB LED Illumination in Confocal Imaging for Cellular Imaging. Jurnal OptoElektronik, 1–3. https://doi.org/10.53655/joe.z7420y

Issue

Volume 4 (2025)

Section

TMP2025: Regular

License

Copyright (c) 2025 Muhammad Faizul Hadi Abu Kasim, Fazliyana 'Izzati Za'abar, Mohd Hadri Hafiz Mokhtar, Norhana Arsad, Mohd Norzaliman Mohd Zain

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